ElleDee
Member
I am not convinced vacuuming the substrate is the main issue. It's not that much sand and I would think that there would be other evidence such as a bad smell, ammonia spike, or longer lasting fish weirdness. I think the culprit is the more than 10x the appropriate Prime dose. Seachem says up to 5x is ok in an emergency and we're well outside that margin of safety in this case. (Unless I'm doing my math wrong... It's early here and bad math can strike the best of us.)