Fe Gluconate usually doesn't last more than an hour in solution. It will then precipitate. It's also photosensitive and will degrade with light hence why it's better to dose it when lights off. As far as I know plant still absorb nutrients even during the dark period. Finally, Fe gluconate is also more suitable when you have softer water. For harder water DTPA Fe and EDDHA Fe are usually better.
Using softer water will make everything much more easy.
This is an interesting note (about the gluconate) - I will bear it in mind moving forward. I think I'm just going to go with your suggestion of softer water and see what happens, I feel more confident about that idea than continuing down the iron route, I've spent many months trying that so far.
To me the plants that are higher up in the water column seem relatively healthy, that includes the frogbit (in some of the photos.)
I'm a bit confused about the frogbit, because I spoke to Darrel
@dw1305 privately and he said the new growth does look a bit pale. I don't have much experience with the plant, so I can't really comment on what it 'should' look like. It doesn't help that it seems to look different in different lights.
I just wonder if this gas is reaching all the areas of the tank.
Lower down in the tank we can see, or you explain that the plants are dropping their lower leaves, holes are appearing in leaves, some leaf edges are disintegrating and bba is present on some leaf edges.
In my limited experience the above issues are generally related to poor Co2 distribution.
This is a valid comment, and is one I have taken into account myself. I actually used to use a standard outtake pipe but swapped to a spray bar after reading many of Clive
@ceg4048 's posts. My hygrophilia compacta at the front of the tank displays this loss of lower leaves and pinholes, and yet these very lower leaves that it loses are constantly visibly swaying in the flow, so I would struggle to accept it being flow in that specific plant. I also topped my limnophilia (which loses it's lower leaves), and replanted the cutting at the front of the tank where I can see the lower leaves move in the flow, and it still loses them too.
I'm also otherwise confused about the yellowing / chlorosis - from my understanding I don't think a CO2 deficit causes chlorosis, although I have read many mixed posts on this subject debating it. I can see that a CO2 deficit can cause plants to consume their older leaves, turning them yellow and then necrotic /translucent, but I haven't seen much evidence that it causes chlorosis in new growth that it keeps around.
This is a confusing one
I'm going to go with lowering the dKH and see what happens. I really don't see what else I can do with the CO2.