Hi all,
I am aware of a few reasons why NO3 values may be incorrect such as:
[1] Some test kits reporting 'nitrate' as 'nitrate nitrogen'. Then, needing to multiply the result by 4.43.
[2] Not shaking one of the reagents adequately.
[3] Using test strips instead of liquid tests.
But I would be keen to learn more.
In terms of tests that require a colour change the problem is really that ~ all <"
nitrate compounds are soluble">.
This means that we need to reduce the nitrate (NO3) to nitrite (NO2), and then combine that NO2 with a second reagent to give a coloured compound. From <"
https://archive.epa.gov/water/archive/web/html/vms57.html">
The cadmium reduction method is a colorimetric method that involves contact of the nitrate in the sample with cadmium particles, which cause nitrates to be converted to nitrites. The nitrites then react with another reagent to form a red color whose intensity is proportional to the original amount of nitrate. The red color is then measured either by comparison to a color wheel with a scale in milligrams per liter that increases with the increase in color hue, or by use of an electronic spectrophotometer that measures the amount of light absorbed by the treated sample at a 543-nanometer wavelength. The absorbance value is then converted to the equivalent concentration of nitrate by using a standard curve.
We don't tend to use cadmium (Cd) any more, but all these types of colourimetric tests work in the same way, just with less toxic reagents.
Other than the factors you've mentioned, issues can come with interference from other monovalent anions, this also affects
<"ion selective electrodes">.
My suspicion is that a lot of the variability is really down to people not diluting the initial sample of tap water with RO.
You need to do more tests, but you just serially dilute the sample down until you don't get a colour change. At that point, using the conversion factor that you got from the equation for your standard curve, you multiply the last positive NO3 value you got by the dilution factor.
I've actually done this with (someone else's tank water, using the MQuant strips) and the initial ~25 ppm NO3 reading, went following dilutions, to about 200ppm NO3. I wouldn't rely on the 200 ppm as accurate, but I'd be willing to bet the actual value was nearer 200 ppm than 25 ppm.
As a disclaimer the strips were all I had to hand, so I don't have an accurate value, and I would agree that there are better options for the aquarium.
cheers Darrel