My RCS in my 60 litre have stopped breeding for some reason (I never see any females with eggs anymore). I used to have around 5 females with eggs at a time and now I have none! I thought perhaps all my females died (unlikely I know) but I have not seen any dead shrimp and I have seen females with saddles. I have around 40-60 shrimp and nitrates are reasonably low. I have starting dosing with the EI method recently but that has been after the problem started. The other thing it could be could be something to do with me as I started cleaning the internal filter weekly as flow was dropping. Could it have anything to do with that? Cleaning with aquarium water of course, and I have now added an eheim internal to help out. Any advice much appreciated.