Any increase in the pipe length will increase the resistance of the pipe which in turn may decrease the flow, how much it decreases the flow is dependant apon if the flow is being restricted already. Shorter, wider bore pipes give less resistance so will give better flow. Then it depends on the power of the pump.
Can be tricky to now how much it will affect the flow especially with basically the short amount of tubing your adding. Just try and have a slow bend in the tubing and try it, especially if you have the spare tubing already.
Why did you want to have the tubing routed that way?
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